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KMID : 0379119990270010027
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Korean Journal of Mycology
1999 Volume.27 No. 1 p.27 ~ p.31
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Genetic Relationships of Coprinus spp. on the Basis of Sequences in ITS 2 Region
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류진창/Ryu, Jin Chang
김양섭/석순자/성재모/박동석/고승주/송재경/Kim, Yang Sup/Seok, Soon Ja/Sung, Jae Mo/Park, Dong Suk/Go, Seung Joo/Song, Jae Kyeong
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Abstract
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The internal transcribed spacer II regions (ITS II) of the ribosomal DNA gene repeat from Coprinus spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 11 species including Coprinus comatus, C. atramentarius, C. micaceus, C. lagopus, C. cinereus, C. rhizophorus, C. flocculosus, C. radians, and C. echinosporus were compared. The spacer regions of them were 253∼275 nucleotide in length and partially contained 5.85 and 255. The reciprocal homologies of each ITS II sequence among these strains were in the range of 50.6∼100%. According to the analysis of ITS II sequences, Coprinus spp. were classified into three clusters. Cluster I consisted of Coprinus lagopus, C. cinereus, C. echinosporus, C. rhizophorus, C. niveus, and C. alramentarius. Cluster II comprised C. micaceus, C. flocculosus, C. radians, and C, disseminatus. On the other hand C. comatus is in Cluster III even though this species is belonging to the section Coprinus in morphological aspect. These results suggest that Coprinus comatus, which was considered as a type species of the genus Coprinus in morphological classification, gives a doubt of monophyletic evolution and is assumed to be paraphyletic or polyphyletic.
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